Journal: PLoS Pathogens

Article Title: Identification of FAM111A as an SV40 Host Range Restriction and Adenovirus Helper Factor

doi: 10.1371/journal.ppat.1002949

Figure Lengend Snippet: (A) Immunoprecipitations for FAM111A and LT with lysates from U-2 OS cells stably expressing full-length LT (LT) or vector control (V). Whole cell lysate of the U-2 OS cell line stably expressing shRNA-2 (sh) against FAM111A was used as a control for FAM111A antibody specificity and normal rabbit serum (IgG) as an immunoprecipitation control. The indicated proteins were detected by western blot analysis. (B) Immunoprecipitations for FAM111A with lysates from U-2 OS cells stably expressing the LT C-terminus (CT) or vector control (V). (C) Immunoprecipitations of FAM111A and FAM111B in U-2 OS cells expressing LT or mock (M). Levels of FAM111A, FAM111B, LT, and vinculin (VIN) were determined by western blot. (D) Immunoprecipitations of FAM111A on U-2OS, BSC40, and CV-1P cells 48 hours post-infection with wild type SV40 (SV) or mock infected (M). (E) Immunoprecipitation of FAM111A in U-2 OS cells transfected with viral DNA encoding wild type SV40 (WT), K697R acetylation mutant (KR), T701A phosphorylation mutant (TA) and host range mutants HR684 (HR) and dl1066 (dl).

Article Snippet: The following antibodies were used: LT mouse monoclonal antibodies PAb419 and PAb901 ; HA mouse monoclonal antibody HA-11 (Covance); VP1 rabbit polyclonal antibody supplied by N. Christensen (Pennsylvania State University); FAM111A antibodies BL8623 and BL8624, FAM111B antibodies BL8627 and BL8630 and SET antibodies were generated by Bethyl Labs. Antibodies to p107, Cyclin E, CDK2, Cyclin B1 and E2F1 were obtained from Santa-Cruz Biotechnology.

Techniques: Stable Transfection, Expressing, Plasmid Preparation, Control, shRNA, Immunoprecipitation, Western Blot, Infection, Transfection, Mutagenesis, Phospho-proteomics

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: Correlations of FAM111B expression in TMA with clinicopathological characteristics in patients with PAAD.

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: TCGA pancreatic adenocarcinoma patient characteristics.

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Biomarker Discovery, Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: (A) Analysis of FAM111B differences in mRNA expression levels between tumor and normal tissues in the GEPIA2 platform. FAM111B gene was overexpression in tumor than in normal tissues. (*: p < 0.01). (B) Overall survival (OS) analysis demonstrated that high expression of FAM111B was associated with a worse prognosis ( p < 0.01); (C) The expression of FAM111B correlated significantly with the patient clinical grade ( p < 0.05). (D) Enrichment plots from gene set enrichment analysis (GSEA). (E) multivariate analysis of the correlation of FAM111B expression with OS among PAAD patients.

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Expressing, Over Expression

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: Univariate analysis and multivariate analysis of the correlation of FAM111B expression with OS among PAAD patients.

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: (A) Effect that overexpression of FAM111B and knockdown of FAM111B on apoptosis of SW1990 cells was analyzed by flow cytometry experiments. (B) The effect that overexpression of FAM111B and knockdown of FAM111B on the proliferation of SW1990 cells was detected by CCK8 assay at 24 h and 48 h. (C) Cell scratch assay to analyze the effect of overexpression of FAM111B and knockdown of FAM111B on the migration of SW1990 cells. (D) Overexpression of FAM111B promoted invasion of SW1990 cells, while knockdown of FAM111B inhibited invasion of SW1990 cells using Transwell invasion assay. Data are expressed as mean ± standard deviation. *: p < 0.05; **: p < 0.01; ***: p < 0.001.

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Over Expression, Knockdown, Flow Cytometry, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Standard Deviation

Journal: Journal of Clinical Laboratory Analysis

Article Title: Clinicopathological features, prognostic significance, and associated tumor cell functions of family with sequence similarity 111 member B in pancreatic adenocarcinoma

doi: 10.1002/jcla.24784

Figure Lengend Snippet: (A) Full view of the immunohistochemistry staining of FAM111B in the TMA cohort with 115 pancreatic carcinoma tissues and 18 normal tissues. (B) The IRS value of FAM111B protein in pancreatic carcinoma tissues was significantly higher than that in normal tissues. **: p < 0.05. (C) Representative images of FAM111B immunostaining on the pancreatic carcinoma tissues and normal tissues microarray (original magnifications ×100 and ×400).

Article Snippet: The TMA slides were incubated overnight at 4°C with rabbit polyclonal antibody against FAM111B (Cat No: 14247‐1‐AP, 1:50, Proteintech, USA).

Techniques: Immunohistochemistry, Staining, Immunostaining, Microarray